Download Culture of Human Stem Cells (Culture of Specialized Cells) by R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach PDF

By R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach

This book collects the simplest and state of the art equipment and protocols for deriving and culturing human embryonic and grownup stem cells—in one convenient resource.
This groundbreaking book follows the culture of past books within the tradition of specialised Cells Series—each equipment and protocols bankruptcy is laid out precisely just like the subsequent, with stepwise protocols, preceded through particular standards for that protocol, and a concise dialogue of tools illustrated by means of information. The editors describe a constrained variety of consultant suggestions throughout a large spectrum of stem cells from embryonic, infant, and grownup tissue, yielding an all-encompassing and flexible consultant to the sector of stem cellphone biology and culture.
The e-book encompasses a finished checklist of providers for all apparatus utilized in the protocols provided, with web content on hand in an appendix. also, there's a bankruptcy on quality controls, and different chapters masking felony and moral concerns, cryopreservation, and feeder layer culture. This textual content is a one-stop source for all researchers, medical scientists, academics, and scholars fascinated with this significant region of research.

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Extra resources for Culture of Human Stem Cells (Culture of Specialized Cells)

Example text

2. Passaging of Mouse Embryo Feeder (MEF) Cells . . . . . . . . . . . 3. Freezing MEF Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3. Cryopreservation of Mouse Embryo Feeder Cells . . . . . . . . . . 4. Thawing MEF Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4. Thawing Cryopreserved Mouse Embryo Feeder Cells . . . . . . . . 5.

1. Outline of hES Cell Differentiation. . . . . . . . . . . . . . . . . . . . . . . . 2. Embryoid Body Formation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14. Generation of Embryoid Bodies from hES Cells . . . . . . . . . . . 9. Sources of Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References .

2. Summary of Embryo Development . . . . . . . . . . . . . . . . . . . . . . . 3. Immunosurgery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6. Derivation of Human Embryonic Stem Cell Lines . . . . . . . . . . . 5. Human Embryonic Stem Cell Propagation . . . . . . . . . . . . . . . . . . . . . . . 1. Culture on MEF Cells and Feeding Routines. . . . . . . . .

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