By Goeddel D.V., Emr S.D., Gold L. (eds.)
The tools offered during this quantity will permit the reader to layout powerful innovations for the expression of cloned genes and cDNAs and should turn out important in fixing the vast majority of expression difficulties one is probably going to come across.
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12, 3937 (1984). 66 B. E. Schoner, R. M. Belagaje, and R. G. Schoner, Proc. Natl. Acad. Sci. A. 83, 8506 (1986). 67 H. A. de Boer and R. A. Kastelein, in "Maximizing Gene Expression" (W. Reznikoffand L. ), p. 225. Butterworth, Stoneham, Massachusetts, 1986. 6s M. Bulmer, Nature (London) 325, 728 (1987). 69 M. Robinson, R. Lilley, S. Little, J. S. Emtage, G. Yarranton, P. Stephens, A. Millican, M. Eaton, and G. Hymphreys, Nucleic Acids Res. 12, 6663 (1984). 70 L. Holm, Nucleic Acids Res. 14, 3075 (1986).
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Mol. Biol. 195, 949 (1987). 60 y. Flashner and J. Gralla, Proc. Natl. Acad. Sci. A. 85, 8968 (1988). 6t M. Mossing and T. Record, Science 233, 889 (1986). 62 H. Kramer, M. Amouyal, A. Nordheim, and B. M011er-Hill, EMBO J. 7, 547 (1988). 50 EXPRESSION IN g . coli  tive internal operator and thus are probably not fully repressible. Therefore, for tightest repression, one probably wants a tight DNA-binding protein that is capable of forming tight higher order structures when interacting with a suitably spaced operator element of optimal sequence.