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By A. T. Annunziato, R. D. Smith, S. K. Hanks, R. L. Seale (auth.), Philip Skehan, Susan J. Friedman (eds.)

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34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. Annunziato, A. , and Seale, R. L. (1983). J. Bio1. Chern. 258, 12675-12684. Frado, L. l~nnunziato, A. , Seale, R. , and Woodcock, C. L. , submitted. Annunziato, A. , and Seale, R. , submitted. , and Groudine, M. (1976). Science 193, 848-856. Lawson, G. , Knoll, B. , March, C. , and O'Malley, B. W. (1982). J. Biol. Chern. 257, 1501-1507. , and Felsenfeld, G. (1982). J. Biol. Chern. 257, 7730-7736. Jahn, C. lo, Hutchinson, C. A. III, Phillips, S.

However, tyrosine modifications resulted in a virtually complete loss of HDP activity as measured by: (a) isothermal unwinding of poly [d(A-T) -d(A-T)); (b) lowering of the melting temperature of DNA; and (c) DNA-binding activity. Figure 4 shows that nitration of less than 2 tyrosines per subunit resulted in loss of melting activity; there was also a concommitant loss of DNA-binding activity (data not shown). Furthermore, nitration of HDP under similar conditions but in the presence of either single stranded DNA or poly d(T) failed to modify the protein, as measured by spectrophotometry and amino acid analysis, and almost complete retention of activity was observed.

Cell 17. 835848. 57. , and Chambon, P. (1980). Prog. Nucleic Acids Res. Mol. Sial. 24, 1-55. 58. , Sheffery, M:-: Krauter, K•• Darnell, J. E. , and Marks, P. A. (1984). J. Mol. B1ol. R, 437-450. CHROMATIN CHANGES IN SKELETAL MUSCLE CELLS DURING THE FUSION TO MYOTUBES O. TOROK, H. ALTMANN * Department of Biology, Sernrnelweis University of Medicine, Budapest, Hungary * Institute of Biology, Research Centre Seibersdorf, Austria ABSTRACT Differentiation of cells is connected to changes in chromatin structure.

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