Download Immobilisation of DNA on Chips I by María Isabel Pividori, Salvador Alegret (auth.), Christine PDF

By María Isabel Pividori, Salvador Alegret (auth.), Christine Wittmann (eds.)

M.I. Pividori, S. Alegret: DNA Adsorption on Carbonaceous fabrics .-

F. Luderer, U. Walschus: Immobilization of Oligonucleotides for Biochemical Sensing through Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces.-

Y. Okahata, T. Kawasaki: guidance and Electron Conductivity of DNA-Aligned solid and LB motion pictures from DNA-Lipid Complexes.-

A. del Campo, I.J. Bruce: Substrate Patterning and energetic thoughts for DNA Chip Fabrication.-

D.V. Nicolau, P.D. Sawant: Scanning Probe Microscopy experiences of Surface-Immobilised DNA/Oligonucleotide Molecules.-

A. Guiseppi-Elie, L. Lingerfelt: Impedimetric Detection of DNA Hybridization: in the direction of close to sufferer DNA Diagnostics

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Such different responses are to be expected considering the different composition and roughness of the various graphite leads containing various insulating polymeric binders and clays besides graphite [116]. However, these non-graphite constituents do not yield a background peak. In the view of the composite nature of the leads, these differences could reflect the differences in the adsorption properties of the nucleic acid, in the kinetics of DNA oxidation processes, or in the effective surface area (roughness) [114].

Many molecules interacted together by hydrogen bonding during equilibration on the substrate, and hydrophobic interactions and van der Waals forces also contributed to adsorption of DNA on the HOPG electrode [16]. 0 PBS [65]. 3 presented a self-assembled lattice that was more relaxed and extended on the surface. 5 [65]. Owing to these characteristics, PG has been extensively used for the adsorption of DNA and its derivatives. DNA was successfully adsorbed on PG by dry-adsorption at 100 ◦ C [67].

Therefore, an overview of self-assembled monolayers based on gold surfaces and thiol compounds on the one hand and silanization on silica surfaces on the other hand will be given in the following sections. 2 Formation of SAMs from Thiol Compounds on Gold Surfaces A well researched and popular class of monolayers is based on the strong adsorption of thiols (R – SH), disulfides (R – S – S – R) and sulfides (R – S – R) onto metal surfaces. Although thiols, disulfides, and sulfides strongly align with a number of different metals like gold, silver, platinum, or copper, gold is usually the substrate of choice because of its inert properties and the formation of a well-defined crystal structure.

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