By David A. Cheresh
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1. The x-axis has been converted from radial distance to shear stress based on Eq. 1 as part of the data transform. X ¼ 0 is the center with minimal shear stress. Increasing the rate of rotation increases the slope of the shear gradient. There is also generally a decrease in density at the edge even without spinning due to surface tension. Hence, values of t50 that fall in the outer 25% of the gradient should be treated as suspect, and duplicate discs should be spun at a higher speed to validate the values obtained (Fig.
Examples of plots obtained from the spinning disc analysis are shown in Fig. 3. The data points represent normalized cell densities for the 61 fields counted, and the lines represent the curve-fit. The critical parameters to focus on are t50 and R2, which are obtained from the curve-fit, rotation speed, and center count used for normalization. 1. The x-axis has been converted from radial distance to shear stress based on Eq. 1 as part of the data transform. X ¼ 0 is the center with minimal shear stress.
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