Download Membrane Processes: Molecular Biology and Medical by J. M. Wrigglesworth (auth.), Gheorghe Benga, Harold Baum, PDF

By J. M. Wrigglesworth (auth.), Gheorghe Benga, Harold Baum, Fred A. Kummerow (eds.)

The burgeoning curiosity in biomembranes lately has been such that "membranology" is now virtuMtyasubject in its personal correct, slicing vertically, because it have been, during the strata of traditional disciplines from arithmetic and physics, via chemistry, to biology. The very scope of the subject is therefore so daunting that it really is tempting to regard it in simple terms at one stratum of this hierarchy, be it the biophysics of phospholipid bilayers or the biochemistry of interactions on the mobilephone floor. Such an process is completely legitimate, really between experts with universal pursuits. although, this technique does current a distorted point of view to the newcomer to the sphere, and, extra considerably, it fails to stimulate pass fertil­ ization of principles between staff on the quite a few disciplinary degrees. for instance, as in all parts of molecular biology, the clinicians are usually ignorant of the contributions to their difficulties that would be made through the appliance of extra uncomplicated wisdom and methods. Conversely, biochemists or biophysicists could be blind to the prevailing sensible difficulties to which they could tackle their expertise.

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FEBS Lett. 50,98-101. , and Metcalfe, LC. (1974). Reconstitution of a calcium pump using defined membrane components. Proc. Nat!. Acad. Sci. USA 71,622-626. , and Saraste, M. (1981). Cytochrome Oxidase-A Synthesis. Academic Press, London. M. (1980). A procedure for membrane-protein reconstitution and functional reconstitution of the anion transport system of the humanerythrocyte mem brane. Biochem. J. 189, 35-44. S. M. (1976). Adsorption of glyceraldehyde 3-phosphate dehydrogenase on condensed monolayers of phospholipid.

Biochim. Biophys. Acta 101, 165-171. Hosaka, Y. and Shimizu, K. (1977). Cell fusion by Sendai virus. In Virus infection and the cell surface. Cell Surface Reviews, Poste, G. , eds. , Amsterdam, vol. 2, pp. 129-155. A. (1973). Interactions of membrane phospholipids and fusogenic lipids. Biochim. Biophys. Acta 332, 1-10. A. (1976). Interkingdom fusion between human (HeLa) cells and tobacco hybrid (GGLL) protop1asts. Science 193, 401-403. A. and Me1chers, G. (1973). The effect of high pH and calcium on tobacco leaf protoplast fusion.

Recently, Honda et al. (1981) have reported that the fusion of human erythrocytes induced by poly(ethylene glycol)-6000 is decreased or even completely prevented when the polymer is recrystallized from chloroform and diethyl ether and/or dialyzed against distilled water. These workers have suggested that 2. 1. 6 PEG (mol wt) SOURCE: Blow et at. (1978). FEBS Lett. 94, 305-310. Reproduced with permission. aCell fusion was measured after a 30-60-s treatment of the cells with PEG. The cells were seeded at 2 X lOs cells onto 50 mm diam Petri dishes, each containing one 2 X 2 mm glass cover slip, and the values given are means of the fusion observed in replicate dishes, ± standard error of the mean (SEM) where appropriate, with the number of dishes shown in parentheses.

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