Download Molecular Pharmacognosy by Lu-qi Huang (auth.), Lu-qi Huang (eds.) PDF

By Lu-qi Huang (auth.), Lu-qi Huang (eds.)

"Molecular Pharmacognosy” discusses the appliance of molecular biology in source technology and authentication of conventional chinese language drugs (TCM). This booklet studies the newest advancements in pharmacognosy, introduces a chain of latest perspectives and insights, provides the hotspots and concentration of the sector of analysis on molecular pharmacognosy, and predicts a brand new path of research at the source technological know-how of TCM. additionally, the e-book additionally presents an open communications platform for the advance of molecular pharmacognosy. This e-book is meant for biomedical scientists and researchers within the fields of molecular biology, conventional medication and typical pharmaceutics.

Professor Lu-qi Huang is Director of the taking part Centre of the area wellbeing and fitness association for standard medication (Chinese Materia Medica) and Vice-Chairman of the Australia chinese language organization for Biomedical Sciences Inc.

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30 Lu-qi Huang et al. One of the most reliable methods for identification of Chinese medicinal materials is by analyzing DNA. In terms of the mechanisms involved, DNA methods can be classified into three types, namely, polymerase chain reaction (PCR)-based, hybridization-based, and sequencing-based methods. 1 PCR-Based Method PCR-based methods use amplification of the region(s) of interest in the genome; subsequent gel electrophoresis is performed to size and/or score the amplification products.

15 mmol · L−1, and template DNA 50–200 ng. PCR amplification was conducted on AB I9700 amplification instrument. Primer screening and optimization of PCR conditions were conducted on all APAPD primers first using typical materia medica (usually four samples) to be identified. Preliminary screening was conducted following RAPD general procedures: predenaturation at 94 °C for 5 min, followed by 40 cycles: denaturation at 94 °C for 45 s, annealing at 37 °C for 1 min, and extension at 72 °C for 1 min 30 s, with a final extension at 72 °C for 5 min after 40 cycles.

The major technology and platform of systems biology consist of genomics, transcriptomics, proteomics, metabonomics, interactomics, and phenomics [15]. Genomics is about genome mapping (including genetic map, physical map, and transcription map), nucleotide sequences analysis, gene mapping, and gene function analysis to all genes of a species. And the common analysis methods of transcriptomics are differential display, gene chip, EST, MPSS, cDNA-AFLP, etc. [16, 17]. Recently, the German scientist Marc Sultan [18] utilized deep sequencing to get a brand-new view of the human transcriptome, and it is expected to apply to transcriptomics of other species.

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