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By Doddapuneni Krishna Rao

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The discovery of Diflucan (fluconazole) was a major landmark in the pharmaceutical industry, as it was the first antifungal drug to be developed that could be used both orally for minor infections such as Candidiasis and intravenously for more serious systemic infection like cryptococccal meningitis. Diflucan tablets are available in 50, 100, 150, and 200 mg of fluconazole and its formulation contains microcrystalline cellulose, dibasic calcium phosphate anhydrous, povidone, croscarmellose sodium, FD and C Red No.

6. 35 M ascorbate, pH 5. 05. The quenched solution was washed and concentrated at 5C using a D2O buffer consisting of 7 mM citrate and 7 mM ascorbate, pH 5. The samples were then subjected to NMR analysis by recording magnitude COSY and TOCSY spectra in 400 MHz (Bruker) or 600 MHz (Varian) spectrometers. Proton occupancies at individual amide sites were calculated by using the cross peak intensities of a control unfolded sample whose amide sites were labeled maximally. Rates were extracted from plots of proton occupancies as a function of refolding time.

Prakash Prabhu and Abani K. Bhuyan melt to the initial unfolded state to have a chance to refold to the correct native state. 2. INTRODUCTION Emerging ideas of landscape perspective or the funnel model [1-6] were first used to interpret both two-state folding kinetics and accumulation of misfolded structures on the basis of size, slope, and roughness of energy landscapes [7, 8]. Corroboration between experimental and simulation results has also been shown regarding the fractional  values for CI2 [9].

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