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By Shashi Bhushan Gogia

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For group AMD3100, the same dose of dMdCs were incubated for 30 minutes at 37°C with 5 µg/mL AMD3100 (Sigma-Aldrich, Missouri, USA), then were washed in phosphate buffered saline and transplanted. All animals were fed with commercial laboratory food and purified tap water and housed under standard conditions. Three days after transplantation, rats were sacrificed and four rats from each group were used in one of the following experiments. Quantitative Real-Time TaqMan PCR The amount of transplanted male dMSCs in bone marrow and skin of female recipient was determined by quantitative real-time TaqMan PCR for rat SRY.

Byk, A. Nagler, H. Ben-Hur, A. Many, L. Shultz, O. Lider, R. Alon, D. Zipori, and T. Lapidot, Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4, Science 283, 845–848. (1999). D. Abbott, Y. Huang, D. Liu, R. S. J. Giordano, Stromal cellderived factor-1alpha plays a critical role in stem cell recruitment to the heart after myocardial infarction but is not sufficient to induce homing in the absence of injury. Circulation 110, 3300-3305 (2004). [7] Kollet, S. Q. Chen, J.

Nature, 418, 41-49. , and Ruohola-Baker, H. (2005). Stem cell division is regulated by the microRNA pathway. Nature, 435, 974-978. , and Vogel, J. (2006). Experimental approaches to identify non-coding RNAs. Nucl. Acid. , 34, 635–646. [15] Storz, G. (2002). An expanding universe of noncoding RNAs. Science, 296, 1260– 1263. F. (2005). Mapping of conserved RNA secondary structures predicts thousands of functional noncoding RNAs in the human genome. Nat. , 23, 1383–1390. , and Szostak JW. (1990). In vitro selection of RNA molecules that bind specific ligands.

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