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By Karl Schügerl, K.-H. Bellgardt, S.R. Gerlach, R. King, P. Krabben, J. Nielsen, G.C. Paul, M.-N. Pons, K. Schügerl, D. Siedenberg, C.R. Thomas, H. Vivier

The cutting-edge of the bioengineering features of the morphology of microorganisms and their courting to approach functionality are defined during this quantity. fabrics and strategies of the electronic picture research and mathematical modeling of hyphal elongation, branching and pellet formation in addition to their program to numerous fungi and actinomycetes through the creation of antibiotics and enzymes are awarded.

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A. 17a,b. Pellet growth characteristics in A. nigerbatch culture: a cell dry weight and the mean equivalent diameter of the whole pellet and core; b mean core area and mean core circularity 34 G . C . P a u l . C. R. 4 0 150 F e r m e n t a t i o n T i m e (h) Fig. 17 c, d. c m e a n a n n u l a r a r e a a n d m e a n a n n u l a r f u l l n e s s ; d r a t i o o f m e a n c o n v e x a r e a o f c o r e t o m e a n c o n v e x a r e a o f t h e w h o l e p e l l e t [14] equivalent diameter and mean core circularity of an industrial strain of A.

Chrysogenum spores. The other two parameters were Table 5. 2 Characterisation of Mycelial Morphology Using Image Analysis 37 settings for the maximum spore diameter and the maximum length of a germ tube. These parameters are used to distinguish between spores, germ tubes and debris. It was found that the accuracy of the measurements could be improved if different values of these parameters were used in the early and late stages of germination (Table 5). This was particularly important when large amounts of debris and media particles were present.

28. 28. C. Paul. R. Thomas Table 9 gives the results for a sample taken from a fed-batch P. chrysogenum fermentation illustrating the information generated by the image analysis method on a typical sample. Figures 29 and 30 show the distributions of vacuole volume and vacuole circularity for a number of fermentation samples across a fed-batch fermentation. The cumulative distribution of vacuole volumes for three samples (Fig. 31) shows the increasing proportion of larger vacuoles with the duration of fermentation.

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