By S. Patai, Z. Rappoport
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Extra resources for The Chemistry Of Halides, Pseudo-Halides And Azides [Parts 1, 2]
4B). Dexamethasone interacts with a rat glucocorticoid receptor-LexA DB hybrid and FK506 interacts with its binding protein, FKBPI2, fused to an AD. The small organic hybrid ligand thus bridges the two chimeric proteins, thereby leading to reporter activation. A cDNA encoding 48 W. Kolanus FKBPl2 was recovered in a library screen performed in the presence of the small hybrid ligand. The CID technology has been used in mammalian and in yeast experiments. Its combination with the two hybrid system will likely have a variety of practical applications.
UAS 5-FOA resistance, growth URA3 Fig. 2_ The reverse two hybrid system, variant I: interaction of two fusion proteins leads to transcription of the URA3 gene. the product of which is toxic if the cells are grown on 5-FOA. Expression of an inhibitor leads to 5-FOA resistance and therefore to cell growth principle be possible to provide other modifying factors to facilitate analysis of post-translational or allosteric regulations (Fig. 4A). g. protein phosphatase) as baits to screen for bona fide in vivo substrates (FLINT et al.
STANGER et al. (1995) have identified the RIP protein as a cytoplasmic ligand for the FAS receptor. The FAS system is involved in the regulation of programmed cell death. RIP was characterized by an important feature which separated it from the pool of primary interactors: it lacked the ability to bind to the lpr mutant of the intracellular tail of F AS. This mutation had previously been implicated in a severe dysfunction of the immune system. Once an interaction between two proteins in the two hybrid system has been established, then specific interaction domains can be investigated.