Download The Human T-Cell Receptor Repertoire and Transplantation by Peter J. van den Elsen Ph.D. (auth.) PDF

By Peter J. van den Elsen Ph.D. (auth.)

these analyses it turned transparent that the MHC category I molecule com­ prised a unique groove at the exterior aspect of the molecule. the edges of the groove are shaped by way of the a-helical buildings of the a and a 1 2 domain names and a ground that is shaped by means of eight anti-parallel thirteen strands. some of the polymorphic residues, as made up our minds from DNA series research, are localized inside those a-helices and 13-plated sheets in the groove. extra importantly, those analyses additionally published the presence of elec­ tron-dense fabric within the groove. This fabric used to be accordingly iden­ 568 10 tified as a linear peptide of 8-10 amino acids lengthy. • •- excessive resolu­ tion crystallographic analyses of the category I MHC constitution have printed the lifestyles of so-called wallet in the grooves of the MHC classification I molecules. those wallet certain A-F, exhibited allele-specificity and are without delay concerned about the binding of the peptide, essentially via interplay with the dominant anchor residues as present in MHC type I linked pep tides. 6,7,9,11 the category II MHC antigens consist at the mobile floor of a 34 kD a sequence non-covalently linked to a 28 kD thirteen chain. With the excep­ tion of the DR a-chain, all different MHC type II a and thirteen chains are poly­ morphic.

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Extra info for The Human T-Cell Receptor Repertoire and Transplantation

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CD8 1 Pair 4 8 ~ ~ ;; ii E . ~ . a: D. 15 10 5 0 10 15 V~14 ~ ! ;; . C04 I 4 B. C04 A. 3 6 I. CD8 I 2 Pair 1 Pa1r 2 3 lh Va12 1 2 Pair 1 4 Va17 ~ ~ ;; E • ii ~ a: . 0 1 (l Lll V~12 I c. _:.. e.... E c: o :J -~ ...!!! 6 ~I Fig. 10. 10a and 2. 1Ob represent significant skewing to the CD4• and coa· subsets respectively for TCRA V gene families. 001) 2. 004). 48 The Human T-Cell Receptor Repertoire and Transplantation from FCB 11, FCB 13, and APBMC (Fig. 14); similar observations were made for the recombinations cloned from the other fetal and adult tissues.

J Immunol 1992; 149:2864-71. 39. Hammer J, Takacs B and Sinigaglia F. Identification of a motif for HLA DR1 binding peptides using M13 display libraries. J Exp Med 1992; 176:1007-13. 40. Hammer J. Valsasnini P, Tolba K et al. Promiscuous and allele-specific anchors in HLA-DR-binding peptides. Cell 1993; 74:197-203. 41. Falk K, Rotzschke 0, Stevanovi S et al. Pool sequencing of natural HLADR, DQ and DP ligands reveals detailed peptide motifs, constraints of processing and general rules. Immunogenetics 1994; 39:230-42.

This approach allows us to determine the relative TCRAV and TCRBV gene usage frequencies among PBMC. 2. 1. Almost all TCRAV and TCRBV gene segments tested for could be detected among PBMC of these individuals. The amounts of PCR product of each individual TCRAV and TCRBV gene family varied among the different individuals used in our analyses. In general the TCRAV2, V3, V8, V12 and V16 were the most frequently employed TCRAV-gene families. In a similar fashion the TCRBV2, V3, V4, V5, V6, V7, V8, V14 and V19 genes were in general dominant among PBMC derived T-lymphocytes.

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